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KMID : 0352120110270010034
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Journal of Kyung Hee University
2011 Volume.27 No. 1 p.34 ~ p.42
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Poly-r-Glutamic Acid Modifies Cytokine Release In Vitro
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Jeong Ki-Heon
Moon Sung-Hyuk
Choi Jeong-Hwee
Lee Jin-Woo
Kim Nack-In
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Abstract
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Background: Poly-γ-Glutamic acid (γ-PGA) is a safe and biodegradable polymer that is produced by Bacillus subtilis. Recent research showed that γ-PGA can be used as an immune-stimulating and anti-tumor agent. But the mechanism of its actions is still unexplored.
Objective: To investigate the further define the potential of γ-PGA as a modifier of monocyte activity.
Methods: Peripheral blood mononuclear cells (PBMNC), obtained from three healthy volunteers, were incubated after treated with lower molecular γ-PGA (1000 Da) and higher molecular γ-PGA (5000 kDa). The levels of the cytokines IL-1β, IFN-γ and TNF-α were measured. We then examined the levels of these inflammatory cytokines after treated with
lipopolysaccharide (LPS) in the presence or absence of γ-PGA.
Results: Low or high molecular γ-PGA alone induced release of IL-1β, IFN-γ and TNF-α significantly. High molecular γ-PGA induced more cytokines response than low molecular γ-PGA. LPS induced IL-1β, IFN-γ and TNF-α synthesis was enhanced significantly. This effect was augmented significantly by costimulation with LPS/high molecular γ-PGA versus LSP alone.
Conclusion: These results suggest that γ-PGA, especially high molecular γ-PGA, play a role in immune response. As a potent immune modifier, PGA contributes to stimulation of immune responses.
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KEYWORD
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Poly-γ-glutamic acid, Interleukin 1β, Tumor necrosis factor-α, Interferon-γ
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